电离辐射通过转化生长因子-β-介导的上皮-间质类比来促进癌细胞的侵袭迁移

Int J Radiat Oncol Biol Phys 2011 Dec;81 (5): 1530-7. [IF:4.503]Ionizing radiation promotes migration and invasion of cancer cells through transforming growth factor-Beta-mediated epithelial-mesenchymal transition.Zhou YC , Liu JY , Li J , Zhang J , Xu YQ , Zhang HW , Qiu LB , Ding GR , Su XM , Mei-Shi , Guo GZ .Department of Radiation Oncology, Xijing Hospital Fourth Military Medical University, Xi'an, China; Department of Radiation Medicine, College of Preventive Medicine, Xijing Hospital Fourth Military Medical University, Xi'an, China.第四军医大学西京医院放射科

AbstractTo examine whether ionizing radiation enhances the migratory and invasive abilities of cancer cells through transforming growth factor (TGF-β)-mediated epithelial-mesenchymal transition (EMT). Six cancer cell lines originating from different human organs were irradiated by (60)Co γ-ray at a total dose of 2 Gy, and the changes associated with EMT, including morphology, EMT markers, migration and invasion, were observed by microscope, Western blot, immunofluorescence, scratch assay, and transwell chamber assay, respectively. Then the protein levels of TGF-β in these cancer cells were detected by enzyme-linked immunosorbent assay, and the role of TGF-β signaling pathway in the effect of ionizing radiation on EMT was investigate by using the specific inhibitor SB431542. After irradiation with γ-ray at a total dose of 2 Gy, cancer cells presented the mesenchymal phenotype, and compared with the sham-irradiation group the expression of epithelial markers was decreased and of mesenchymal markers was increased, the migratory and invasive capabilities were strengthened, and the protein levels of TGF-β were enhanced. Furthermore, events associated with EMT induced by IR in A549 could be reversed through inhibition of TGF-β signaling. These results suggest that EMT mediated by TGF-β plays a critical role in IR-induced enhancing of migratory and invasive capabilities in cancer cells.

摘要 ：阐述远红外两条路线是否可通过反转成生长因子-β（TGF-β）-介导的内膜-间质反转 (EMT)来促进癌受体质的侵袭迁移。用到年均2Gy(60)Coγ两条路线太阳光源自全人类器官的6种癌受体质，记录与EMT相关的变化，这包含分别利用显微镜应用，受体质印迹方法，免疫荧光应用，样试验性和Transwell小室试验性来观察并检测受体质三组织形态，EMT标识，侵袭迁移能够等。采用酶联免疫吸附法检测这些癌受体质中TGF-β受体低水平，利用特别抑制剂SB431542来评估TGF-β信号通路在远红外两条路线EMT中的起到。经过年均为2Gy太阳光的癌受体质中存在间叶受体质的理解，与有假太阳光三组相比其内膜标识减少，间叶受体质标识增加，同时其侵袭移出能够强化，TGF-β受体低水平也提高。有利于发现由A549远红外两条路线可借的EMT可通过对TGF-β信号抑制发生逆转。这些结果表明TGF-β介导的EMT在远红外两条路线可借强化癌受体质侵袭移出能够中起着关键起到。

编辑： lizexiu